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We have identified a nuclear-encoded Hb from plants (GLB3) that has a central domain
similar to the “truncated” Hbs of bacteria, protozoa, and algae. The three-dimensional
structure of these Hbs is a 2-on-2 arrangement of α-helices, distinct from ...
Overexpression of a class 1 Hb (GLB1) protects Arabidopsis thaliana plants from the effects of severe hypoxia. Overexpression of the bifunctional symbiotic
Hb (GLB1S) from Parasponia andersonii in A. thaliana also increases survival after hypoxia. ...
The chromosomal subunits that segregate from each other during chromosome replication
are shown to be uniquely different. An analysis of tritiated thymidine labeled chromosomal
subunits in dicentric chromosomes, generated by isolocus breakage followed by ...
From the types of ring chromosomes induced in x-irradiated Chinese hamster ovary (CHO)
cells, we deduce the existence of switches in the polarity of chromosomal DNA; if
there is a continuous DNA double helix along the full length of the chromosome then
...
Replication patterns of four classes of heterochromatin in the chromosomes of maize
were analyzed. Centromeric heterochromatin, distal heterochromatin of the B chromosomes,
and knob heterochromatin all have asynchronous late replication, knob ...
A highly repeated DNA sequence has been isolated from the maize genome as a satellite
in actinomycin D/CsCl gradients. By using maize stocks differing in their heterochromatin
content we have established that the sequence is a major constituent of one ...
cDNA clones of Adh1, one of two genes encoding alcohol dehydrogenase (ADH; alcohol:NAD+ oxidoreductase, EC 1.1.1.1) in the maize genome, have been isolated. They were derived
from mRNA extracted from anaerobically treated roots of maize seedlings. ...
A 16-base-pair palindrome from the Agrobacterium tumefaciens octopine synthase gene
functions as a constitutive enhancer in plant protoplasts. Degenerate oligonucleotide
mutagenesis provided single base substitutions at every position in the element and
...
Late-flowering ecotypes and mutants of Arabidopsis thaliana and the related crucifer
Thlaspi arvense flower early after cold treatment (vernalization). Treatment with
the DNA demethylating agent 5-azacytidine induced nonvernalized plants to flower ...
Hammerhead ribozyme sequences were incorporated into a tyrosine tRNA (tRNA(Tyr)) and
compared with nonembedded molecules. To increase the levels of ribozyme and control
antisense in vivo, sequences were expressed from an autonomously replicating ...
We have isolated a new hemoglobin gene from soybean. It is expressed in cotyledons,
stems of seedlings, roots, young leaves, and in some cells in the nodules that are
associated with the nitrogen-fixing Bradyrhizobium symbiont. This contrasts with ...
Arabidopsis plants transformed with an antisense construct of an Arabidopsis methyltransferase
cDNA (METI) have reduced cytosine methylation in CG dinucleotides. Methylation levels
in progeny of five independent transformants ranged from 10% to ...
We have tested the hypothesis that the promotion of flowering by prolonged exposure
to low temperatures (vernalization) is mediated by DNA demethylation [Burn, J. E.,
Bagnall, D. J., Metzger, J. M., Dennis, E. S. & Peacock, W. J. (1993) Proc. ...