Fig. 5

FKBP5 mitigates the effect of IFNβ on reducing osteogenesis of BMSCs. A FKBP5 knockdown resulted in amplification of ISG transcription, while overexpression of FKBP5 down-regulated these ISG. B IFNβ up-regulated the expression of ISG of BMSCs. C IFNβ impaired osteogenic potential of BMSCs, as evidenced by reduced ALP staining and ARS (scale bar: 200 μm). D Quantitative comparison of ALP activity and ARS. E IFNβ weakened ALP staining and ARS of BMSCs, but this effect could be alleviated by FKBP5 overpression (scale bar: 200 μm). F Quantitative analysis of Fig. E. G IFNβ down-regulated protein expression of COL1 A1 and RUNX2 of BMSCs, whereas overexpression of FKBP5 relatively upregulated the protein of these two markers. All experiments were performed independently and repeated three times