Fig. 6: Silencing of ETFA and ETFB leads to decreased proliferation and survival in AML cells but not in normal human CD34+ cells.
From: Multi-omics reveals mitochondrial metabolism proteins susceptible for drug discovery in AML
A–F Differences between MOLM-13 cells lentivirally infected with shRNAs targeting ETFA and ETFB or non-targeting control. 72 h post puromycin selection were analyzed by A cell counts at day 2 and day 4, B ability to form colonies in colony-forming units (CFU) assay, C Annexin V and PI staining for percent apoptotic cells, D CD11b expression by flow cytometry, and E survival rate of sub-lethally irradiated NSG mice transplanted with 5 × 105 MOLM-13 cells infected with shRNAs targeting ETFA and ETFB or non-targeting control. F MTT assay following treatment with 0.1–50 nM venetoclax for 72 h. G–I Human PB mobilized CD34+ cells from 3 independent donors were infected with lentivirus expressing scramble control or shRNAs targeting ETFA and ETFB and analyzed 72 h post puromycin selection for G knockdown by western blot, H CD34 expression by flow cytometry, and I ability to form colonies in CFU assay (N = 3).
